2.6. Cell Viability Assays

Cells were seeded into a white-walled, glass-bottomed 384-well plate (Nunc) at a density of 1 × 103 cells per well. The cells were treated with escalating doses of erlotinib, osimertinib or CX-4945 24 h following seeding over a period of 72 h. Cell viability was determined using CellTitre-Glo 2.0 (Promega Corporation) according to the manufacturer’s instructions. Luminescence was scanned and analysed on the FLUOstar Omega Microplate Reader (BMG Labtech, Mornington, Australia). Data were normalised to untreated controls and dose–response curves and drug potency values generated using GraphPad Prism V9 software.