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Acute and preconditioning kainic acid excitotoxicity models in mice

IP Irini Papazian
ET Eleni Tsoukala
AB Athena Boutou
MK Maria Karamita
KK Konstantinos Kambas
LI Lida Iliopoulou
RF Roman Fischer
RK Roland E. Kontermann
MD Maria C. Denis
GK George Kollias
HL Hans Lassmann
LP Lesley Probert
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In an acute kainic acid (KA) seizure model, 2–6-month-old male nTNFR1KO and nTNFR2KO mice, and their respective TNFR1ff or TNFR2ff littermate controls, were injected intraperitoneally (i.p.) with 20 or 24 mg/kg KA according to the manufacturer’s instructions (Tocris Bioscience). In a modified preconditioning KA protocol based on a previously described model [33], mice were injected i.p. with 15 mg/kg KA and subsequently after 24 h i.p. with 20 mg/kg KA. Seizures were scored every 5 min for 90 min, using a clinical seizure scale based on previously described methods [34, 35]. Briefly, mice were scored as follows: 0, no behavioral response; 1, immobility; 2, one or more of the following: head bobbing, whiskers movement, hunching, outstretched forelimbs; 3, rearing and one-sided forelimb spasms; 4, repeated rearing and two-sided forelimb spasms; 5, one or more of the following: convulsions with sideways leaning, jumping and wild running followed by convulsions, death. Animals with a score of 5 were euthanized.

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