2.2.7. Cell Viability Assay

KB, CT-26 and MDA-MB-231 (CD44 overexpressing human breast cancer cells [29]) cells were plated using 96-well plates at a cell density of 1 × 10⁵ cells per well (5 × 10⁴ for CT-26) in 100 μL of cell culture medium. KB and CT-26 were incubated in DMEM (10% FBS, 1% Anti-Anti) and MDA-MB-231 in RPMI (10% FBS, 1% Penicillin) for 24 h (at 37 °C and 5% CO₂). After that, the experiment was carried out. A total of 100 mL PBS with MTT (0.5 mg/mL) was put into a well, then dissolved in DMSO after 3 h, and absorbance at 570 nm was measured.