2.4. Analysis of Immune Cell Infiltration

We used immuneeconv, an R software package, to calculate the immune score, stroma score, and microenvironment score for each of the GLNM regulators based on xCell algorithms [23]. GEPIA2 was used to evaluate the association between PD-L1 and the gene set expression level. The association between tumor-infiltrating immune cells (TIICs) across 33 cancer types and the gene set expression level was estimated via ImmuCellAI [24]. The Spearman correlation test was applied to analyze the correlation between the expression of GLNM regulators and the abundance of different immune cells.