Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.16. Preparation of NGM Agar Plates

MR Malena Rabenau
BD Benjamin Dillberger
MG Madeline Günther
SK Sylvia Krippner
VB Veronika Butterweck
GB Georg Boonen
JD Jürgen Drewe
GE Gunter P. Eckert
CC Carsten Culmsee
request Request a Protocol
ask Ask a question
Favorite

Three grams of NaCl, 2.5 g Bacto™ Peptone (Becton Dickinson, Heidelberg, Germany) and 17 g Bacto™ Agar (Becton Dickinson, Franklin Lakes, NJ, USA) were dissolved in 975 mL distilled water. After autoclaving, 1 mL cholesterol in ethanol (5 mg/mL), 1 mL 1 M CaCl2, 1 mL 1 M MgSO4, and 25 mL 1 M potassium phosphate buffer (pH 6.0) (108.3 g KH2PO4, 35.6 g K2HPO4, H2O to 1 L) were added in this order.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A