Aerosolized Mtb infection of mice

Mice were infected by the aerosol route as previously described (Lee et al., 2020). Frozen bacterial stocks were thawed and sonicated for 1 minute and then diluted into 5 mL of 0.01% Tween-80 in PBS. The diluted bacterial suspension was aerosolized to infect mice using a Glas-Col chamber (Terre Haute). The average number of bacteria delivered into the lung was determined for each experiment by plating lung homogenate from 4-5 mice 24 hours after infection and ranged between 40-150 CFU/mouse.