Two-electrode voltage clamp

PH Panpan Hou
JE Jodene Eldstrom
JS Jingyi Shi
LZ Ling Zhong
KM Kelli McFarland
YG Yuan Gao
DF David Fedida
JC Jianmin Cui
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Microelectrodes (Sutter Instrument, Item #: B150–117–10) were made with a puller (Sutter Instrument, P-97), and the resistances were 0.5–3 MΩ when filled with 3 M KCl solution. Ionic currents were recorded by Two-electrode voltage clamp (TEVC) in ND96 or 100K+ (NaCl was replaced with 98 mM KCl) bath solutions. Whole-oocyte currents were recorded using a CA-1B amplifier (Dagan, Minneapolis, MN) or a GeneClamp 500B amplifier (Axon Instruments, Foster City, CA) with Patchmaster (HEKA) software. The currents were sampled at 1 kHz and low-pass-filtered at 1 kHz. All recordings were carried out at room temperature (21–23 °C).

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