2.8. Colony formation assay

YZ Yumiao Zhao
JS Jiaxin Sun
YL Yin Li
XZ Xiuman Zhou
WZ Wenjie Zhai
YW Yahong Wu
GC Guanyu Chen
SG Shanshan Gou
XS Xinghua Sui
WZ Wenshan Zhao
LQ Lu Qiu
YY Yongjie Yao
YS Yixuan Sun
CC Chunxia Chen
YQ Yuanming Qi
YG Yanfeng Gao
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KYSE150-Vector, KYSE150-TDO2, KYSE450-Vector, or KYSE450-TDO2 cells were seeded into a 6-well plate with 500 cells/well. After 10 days of culture, the cells were fixed with methanol and stained with 0.5% crystal violet, and then the colonies of cells were counted. The results are expressed as the mean ± SEM of three independent experiments.

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