MicroRNA targeting and protein interaction analysis of TaZF-HD genes

MicroRNA (miRNA) has an important function of regulating gene expression, in order to identify miRNAs that target the TaZF-HD genes, we submitted the TaZF-HD gene sequences to psRNATarget (http://plantgrnbb.noble.org/psRNATarget) with default parameters [41]. And Cytoscape software (https://www.omicshare.com/tools/) was used to drew the linkage between the predicted miRNA and corresponding target genes [42]. Protein-protein interaction (PPI) can regulate a variety of biological activities and functions [43]. In order to better understand the biological functions and regulatory network of TaZF-HD genes, the online STRING database(https://string-db.org) was used to predict the PPI network.