Sample and clinical data collection

Between January 2009 and August 2019, 100 intraoperative tissue specimens from 50 patients undergoing OS surgery in the Department of Pathology, Affiliated Hospital of Youjiang Medical University for Nationalities (Baise, China). These included 50 OS samples and 50 normal tissue samples adjacent to the OS samples. The normal tissue in this study refers to the normal bone tissue adjacent to the enlarged resection of the tumor with a distance >5 cm from the tumor edge during surgical resection of osteosarcoma. Patients (28 male and 22 female) were aged between 11 and 80 years, with an average age of 51±5 years. Preoperative National nosocomial infections surveillance system grade (28,29) was observed in five patients, and grade 0 in 45 patients. All study patients were newly diagnosed OS patients, who had received no other treatment before surgery. Postoperative pathological examination results indicated OS, with no other systematic history of malignant tumors. Inclusion criteria were as follows: i) Patients undergoing osteosarcoma surgery admitted to the Affiliated Hospital of Youjiang Medical University for Nationalities from January 2009 to August 2019 were selected and osteosarcoma specimens were collected; ii) patients have a clear pathological diagnosis; iii) patients with primary osteosarcoma; iv) patients were between 10 and 80 years old; v) patients included both men and women; vi) all patients received the same standard treatment regimen for osteosarcoma; and vii) the clinical cases and follow-up data of the patients were complete. The exclusion criteria were: i) Patients who had been treated with high-dose chemotherapy and other anti-tumor drugs before specimen collection; ii) cases complicated with other malignant tumors; iii) patients with severe heart disease; iv) liver and kidney failure before treatment; and v) perioperative death. A log-rank test was used to analyze Mps1 expression and survival rates of 50 clinical patients with OS data. Prior to sample collection, the study was approved by the Medical Ethics Committee of the Affiliated Hospital of Youjiang Medical University for Nationalities (approval no. YYFy-LL-2018-003). All patients or their parents/legal guardians (for patients <18 years old) provided written informed consent and agreed to the use of their samples in this study.