Constant load test

During each experimental trial a constant load test was completed following the ramp test, which occurred after 10 min of light active recovery (pedaling at a work rate no higher than the warm‐up) on the stationary ergometer. During active recovery, the participants were provided a break from the breathing valve, which was reconnected to the participant at least 3 min prior to the start of the constant load test. The constant load test consisted of cycling at a work rate equivalent to either 85% (CL85) or 110% (CL110) of the peak work rate reached during the preceding ramp test. Specifically, in a randomized, counterbalanced cross‐over design, participants were randomized to perform either CL85 or CL110 during the first visit, whereas during the second visit the participant completed the constant load test at the other work rate. Participants were instructed to increase cadence as the resistance on the cycle ergometer increased from that during active recovery to the prescribed intensity. Both constant load tests were performed at a constant work rate until volitional exhaustion. RPE was assessed at the end of the constant load test. The test was terminated at volitional exhaustion (i.e., the participant requesting to stop) or if the participant was unable to maintain his/her RPM despite verbal encouragement.