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The [Ca2+]i in primary pancreatic islet β-cells was determined using the Ca2+-sensitive fluorescent indicator dye Fluo-4 (Sigma–Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions. Baseline fluorescence was determined by averaging 20 images. For this experiment, 25 mM glucose with or without 10 μM BBR was added to the solution at 50 s during the observation. Images were recorded every 5 s.
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