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Histochemical detection of GUS activity was performed according to Jefferson64. Plant tissues were incubated in X-gluc (Biomol, Hamburg, Germany) solution consisting of 0.1 M sodium phosphate buffer pH 7.0, 0.1% Triton-X 100, 0.5 mM K3[Fe(CN)6], 0.5 mM K4[Fe(CN)6] and 10 mM Na2EDTA at 37ºC overnight. After staining, chlorophyll was removed from photosynthetic tissues with 70% (v/v) ethanol.
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