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Oral glucose tolerance test (OGTT) and insulin resistance (IR)

KO Kehinde Samuel Olaniyi
MO Morounkeji Nicole Owolabi
CA Chukwubueze Lucky Atuma
TA Toluwani Bosede Agunbiade
BA Bolanle Yemisi Alabi
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The OGTT was performed 48 h prior to the sacrifice of the rats. The rats had 12-h overnight fast. Animals were loaded with glucose (2 g/kg; po). Blood was obtained from the tail before glucose load and then sequentially at 30, 60, 90 and 120 min. Glycemic levels were determined with a hand-held glucometer (ONETOUCH-LIFESCAN, Inc., Milpitas). Insulin sensitivity was determined using quantitative insulin sensitivity check index (QUICKI), which is expressed as 1/[log (fasting insulin) + log (fasting glucose)73,74, while IR was determined using its surrogate, triglyceride-glucose index, which is expressed as TyG = Ln (TG (mg/dl) × FPG (mg/dl)/2)51,53,75.

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