Male C57BL/6 mice, 8–12 weeks of age, were obtained from the Animal House at the University of Lodz, Lodz, Poland. All animals were housed in standard rodent cages in a climate-controlled room with an ambient temperature of 23 ± 2 °C and 12:12 h light–dark cycle. Animals were fed standard laboratory chow, given water ad libitum, and randomly assigned to control or experimental groups. All animal protocols were approved by the Medical University of Lodz Animal Care Committee (#70/LB83/2017). All efforts were made to minimize animal suffering and to reduce the number of animals used.
Animals were divided into three experimental groups (n = 8):
Control
Mice with acute pancreatitis (AP)
Mice with acute pancreatitis treated with CGA (AP + CGA)
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