Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Quantification of knockdown of drp-1 mRNA by drp-1 RNAi
Ask a
question
Favorite
Wild-type and BW-Htt74Q worms were grown on empty vector (EV) and drp-1 RNAi following the RNAi parental paradigm described in the RNAi methods. Levels of drp-1 and act-3 were quantified through quantitative RT-PCR. Primers were specifically designed to exclude the drp-1 RNA used to induce RNAi (drp-1: L-GAGATGTC GCTATTATCGAACG, R-CTTTCGGCACACTATCC TG). Three biological replicates each with three technical replicates were performed.
copyright: ©2021 Machiela et al.
this is an open access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
