Salinomycin-Loaded High-Density Lipoprotein (S-HDL) Synthesis and Characterization

S-HDL was synthesized using sodium cholate methods.³⁰ An ethanol solution (1 mL) containing 2.7 mg lecithin and 1 mg salinomycin was rapidly injected into 6 mL phosphate buffer (pH = 7.4) via a skin test syringe. After mixing in N₂ for 15 min, phosphate buffer (0.75 mL) containing 2.7 mg sodium cholate, 5 mg rhApoA- I and 2.5 mg rhApoE was added to the lipid mixture by stirring. The mixture was incubated at room temperature for 30 min and then was incubated at 4°C for 12 h, and the components polymerized to form S-HDL. Then, the solution was mixed and added to a 10KD dialysis bag for complete dialysis with a phosphate buffer at 4°C (about 2 days) to remove ethanol and sodium cholate. After dialysis, the solution was filtered by 0.22 μm filter membrane and stored at 4°C. After the synthesis of S-HDL, it was characterized using a Zetasizer Nano ZS dynamic light scattering (DLS) and a transmission electron microscope (TEM).