With the above information, the v0.66839 Toolkit for Biologists integrating various biological data-handling tools (TBtools) was used to generate the Newick (nwk) files with Latin names of species as input files according to the previous methods under the default parameters (Chen et al. 2018). The nwk files were employed to construct phylogenetic trees using the v6 Molecular Evolutionary Genetics Analysis software under the default parameters (Tamura et al. 2013).
The polyploidy events, such as whole-genome triplication (WGT), whole-genome duplication (WGD) and whole-genome sextuplication (WGS), of plant species came from research in Qu et al. (2018).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.