Statistical analysis for ICC was performed using SPSS statistics software version 27. The remainder of statistical analyses were performed using Prism software version 8. Comparison between two groups was evaluated using an unpaired Student’s t-test. Comparisons between more than two groups were evaluated with one-way ANOVA with post-hoc Tukey’s multiple comparison test. Comparisons of more than two groups over time were evaluated with mixed-effects analysis with post-hoc Tukey’s multiple comparison test. Data were presented as mean ± SEM. Threshold for significance was set at p < 0.05.
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