2.5. MTT cell proliferative assay

MTT assay was used to determine the anti-proliferative ability of respective plant formulations post treatment against cancer cell lines. Cancer cells were seeded (10 × 10³ cells per well) in triplicates in 96-well plates overnight and were treated with 25, 50, 100, 200, and 400 µg/ml of plant extracts for a time interval of 24, 48, and 72 h. After respective time points, 20 µl MTT (5 mg/ml) was added to each well followed by incubation for 3 h. The medium of well was then replaced by 100 µl DMSO to dissolve formazan crystals. The optical density of each well was recorded at 570 nm by microplate reader (SPARK^TM 10 M, TECAN, Switzerland). Cell viability was measured using the equation;