The TIMER database (http://timer.cistrome.org/) is a comprehensive web tool that provide automatic analysis and visualization of immune cell infiltration of all TCGA tumors (32, 33). The infiltration estimation results generated by the TIMER algorithm consist of 6 specific immune cell subsets, including B cells, CD4+ T cells, CD8+ T cells, macrophages, neutrophils and dendritic cells. We extracted the infiltration estimation results and assessed the different immune cell subsets between high-risk and low-risk groups (34).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.