Cell Viability Assay

Cells in the logarithmic growth phase were seeded at a density of 1,500 cells/well and cultured overnight. Drugs were administered at 0.1 μL per well. After a 72-h incubation, cell viability was measured using Cell Titer-Glo luminescent cell viability assay kit (Promega, Madison, United States) and luminescence was quantified using Envision Plate-Reader.

Cell viability was also measured by Cell Counting kit-8 (CCK-8) assay (MCE, Shanghai, China). Briefly, cells were seeded at 3,000 cells/100 μL and treated with different concentrations of CDM and ASA for 24, 48, and 72 h. CCK-8 reagent was added and incubated for 3 h. The absorbance at 450 nm was measured using a Multiskan Go Microplate Spectrophotometer (Thermo Fisher Scientific, United States). Cell proliferation inhibition rate was calculated based on the formula: absorbance of (control group − experimental group)/absorbance of (control group – blank group) × 100%.