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Gut motility assays and assessment of stool water content

TA Thibault Allain
EF Elena Fekete
OS Olivia Sosnowski
DL Dimitri Desmonts de Lamache
JM Jean-Paul Motta
DL Dezirae Leger
TF Troy Feener
RR Raylene A. Reimer
AB André G. Buret
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As Giardia-induced diarrhea is associated with increased intestinal motility89, additional experiments measured the effects of the diets on gut motility and stool water content. Before sacrifice, mice from each group (LF, LF + G, HF, HF + G) were placed in individual cages without water and food for 60 min and fecal pellet number was counted every 15 min for each mouse (n = 4 per group). Fecal pellets were collected at 60 min and placed in an empty Eppendorf tube. Fecal pellets were dried overnight at 60 °C and water content of stools was determined by comparing wet/dry weight difference as an indicator of loose or watery stools.

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