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Cells were seeded at 1 × 105 cells per well in a 24-well plate 24 h before transfection. Cells were transfected with pTopflash/pFopflash, pEGFP-β-catenin/pEGFP-C1, and SV40-Renilla plasmids, using jetPEI. Following transfection (48 h), the cells were harvested and subjected to luciferase assay according to the manufacturer’s instructions. In all assays, Topflash activity was normalized to Fopflash activity. Transfection efficiency was normalized using a Renilla luminescence control.
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