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The antioxidant capacity of the brain tissues was evaluated by assaying activities of SOD and CAT, and the levels of reduced glutathione (GSH). SOD activity was estimated using the Superoxide Dismutase Activity Assay kit (Abcam, Tokyo, Japan) according to the manufacturer’s instructions. The activity of CAT was determined by the Catalase Assay Kit (Cayman Chemical, USA) according to manufacturer’s instructions. Reduced GSH levels were analyzed using the GSH Assay kit (Cayman Chemical, USA) in accordance with the manufacturer’s instructions.
Copyright and License information: ©2021 Zhang et al.
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