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Mitochondria microinjection to the mpv17−/− zebrafish embryos

WB Wan-Ping Bian
SP Shi-Ya Pu
SX Shao-Lin Xie
CW Chao Wang
SD Shun Deng
PS Phyllis R. Strauss
DP De-Sheng Pei
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The mitochondria were isolated from wild-type zebrafish liver using a mitochondrial extraction kit (Solarbio, Beijing, China). According to the manufacturer’s protocol, ten wild-type adult zebrafish livers were collected for the isolation procedure. The livers were washed three times with cold PBS, and then homogenized in the extraction buffer. The homogenate was centrifuged at 1000 × g for 5 min, and repeated once. Subsequently, the supernatant was centrifuged at 12,000 × g for 10 min, and the pellets were washed twice with wash buffer. The pellets were dissolved in PBS and the concentration was determined by BCA assay. Finally, the microinjection of mitochondria was done according to the previous description [46].

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