2.4.2. Tail suspension test

Tail suspension test protocol (Porsolt et al., 1977; Yamawaki et al., 2012) was shown as follows: the tail of the experimental mouse was lifted, and the tail was fixed and suspended, so that the mouse was kept upside down, and the head was about 5 cm away from the ground. The immobility time of the experimental mouse was recorded. The total time length of the experiment was 6 min, the data collected in the last 4 min were analyzed in a double-blind manner.