Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

BODIPY 581/591 C11 Lipid Peroxidation Sensor

WO Wataru Otsu
KI Kodai Ishida
NC Naoki Chinen
SN Shinsuke Nakamura
MS Masamitsu Shimazawa
HT Hideshi Tsusaki
HH Hideaki Hara
request Request a Protocol
ask Ask a question
Favorite

The BODIPY 581/591 C11 (Thermo Fisher Scientific) Lipid Peroxidation Sensor assay was performed according to the manufacturer's instructions. Briefly, cells were incubated with 10 µM BODIPY 581/591 C11 for 30 min at 37 °C. For live imaging, the medium was replaced with live imaging buffer [Live Cell Imaging Solution (LCIS, Thermo Fisher Scientific) supplemented with 4.5 g/L glucose and 1% FBS], and then recorded with a 10 × objective lens on a Lionheart FX Automated Microscope (Bio Tek Instruments). The image analyses were performed with Gen5 software (Bio Tek Instruments).

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A