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Five HIV specimens’ full-length pol gene sequences were obtained by means of SGA and Sanger sequencing [5], which are available in the GenBank database (accession nos. MW190061-MW190073). cDNA from specimens 1430, 1133, 1599, 9544, and 4241 was diluted to approximate the SGA condition and PCR cycled. After the PCR product was Ampure cleaned and diluted to 6 ng/μL, 10 μL of that dilution and 5 μL of each of 7 Sanger sequencing primers (5 μmol/L) were subjected to Sanger sequencing. More detailed methods are provided in the Supplementary Materials.
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