In vitro susceptibility (MIC) and fungicidal (MFC) testing.

Broth macrodilution was performed with arthroconidia in tubes for MIC and MFC determinations as previously described (20, 22–24). In brief, the initial inoculum was 10³ arthroconidia/ml with a 2-fold dilution range, starting at 100 μg/ml for FCZ and 20 μg/ml for nikZ, and was incubated at a temperature to approximate human body temperature (35°C to 37°C). The MIC value was determined when the control growth was 4+, and the endpoint was defined as the first tube without growth. MFC was determined by subculturing the isolate on agar and defined as killing ≥96% of the initial inoculum. Both readings occurred after 7 days of incubation.