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Assessing cell viability and apoptosis
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Cell viability was measured using the CellTiter-Glo assay (Promega) 48 hours after NP treatment. Viability was calculated as a percentage relative to the untreated control. Apoptotic cell death after 48 or 72 hours was measured by Annexin V and propidium iodide (PI) staining (MDA-MB-231 cells) or by TMRE staining (MCF-7 cells) followed by flow cytometry (BD Accuri C6).
Copyright and License information: ©2021 This journal is The Royal Society of Chemistry
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