Mice Model of Bone Cancer Pain

A mouse model of bone cancer pain was established as previously reported (Gonzalez-Rodriguez et al., 2017). Briefly, mice were anesthetized with intraperitoneal injection of pentobarbital sodium (20 mg/kg). Next, the right tibial plateau was exposed, and B16-F10 melanoma cells (1 × 10⁵, 5 μl) were injected into the tibia cavity using a microsyringe. The injection site was then sealed with sterile medical glue to prevent the cancer cells from flowing out. Finally, the wound was closed using surgical suture, and erythromycin ointment was applied to the sutured wound to prevent infection. After recovering from anesthesia, the mice were housed separately.