2.7.6. Detection of PC12 Cell Viability by MTT Method

After inoculating PC12 cells in the logarithmic growth phase in a 96-well plate, place them in a 5% CO₂ incubator and incubate for 24 hours. 20 μL of MTT (5 mg/mL) solution was added to all experimental setup groups and continued to incubate for 4 hours with PBS, then washed 3 times, and discarded the culture medium, and 100 μL DMSO was added to each well and shaked at low speed for 10 minutes to fully dissolve the crystals. The experiment includes control group, corticosterone (model) group, low-concentration puerarin group (50 μmol/L puerarin), medium-concentration puerarin group (100 μmol/L puerarin), and high-concentration puerarin group (150 μmol/L puerarin), and each group was treated with 100 μL of 200 μmol/L corticosterone, respectively, for 24 hours except for the control group and then treated with different concentrations of puerarin for 24 hours. Finally, 10 μL of MTT was added to each well, cultured for 4 hours, and then, the OD value was measured at 450 nm.