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Image analysis

YL Yiting Liu
JL Jiangnan Luo
DN Dick R. Nässel
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Specimens were imaged with a Zeiss LSM 780 confocal microscope (Jena, Germany) using 20 ×, 40 × oil, or 63 × oil immersion objectives. Confocal images were processed with Zeiss LSM software for either projection of z-stacks or single optical sections. Images were in some cases edited for contrast and brightness in Adobe Photoshop CS3 Extended version 10.0. For cell size determination, the outline of each cell body was extracted and its area determined using Image J (freely available at http://rsb.info.nih.gov/ij/). For each genotype neurons in 5–15 animals from three independent crosses were measured.

Copyright and License information:  ©2016 Liu, Luo and Nässel.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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