Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Urease activity was measured as described previously (55). Briefly, overnight cultures of bacteria were washed once in sterile saline and adjusted to ∼5 × 109 CFU/ml. Cultures were then diluted 1:10 in filter-sterilized human urine supplemented with 0.001%, wt/vol, phenol red and 500 mM urea and dispensed into the wells of a clear-bottom 96-well plate. Absorbance (OD562) was measured every 30 s for a total of 90 min in a BioTek Synergy H1 reader.
Copyright and License information: ©2021 American Society for Microbiology.
All Rights Reserved.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
