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The infarct volume was determined using 2,3,5-triphenyltetrazolium chloride (TTC) staining. Rat brain samples were collected 7 days after treatment administration and immediately frozen at −20°C for 15 min. The frozen samples were then cut into 2-mm-thick coronal slices and then stained with 2% TTC solution at 37°C for 20 min in the dark. These slices were photographed, and the infarct volume was calculated using ImageJ.
Copyright and License information: ©2021 Yu, Zhang, Zhao, Wan, He and Jin.
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