Quality Control of GHI

According to the Chinese national standard [No. WS-10001-(HD-1506)-2004] promulgated by Chinese Pharmacopoeia Commission and the process of GHI: Each 1,000 ml of GHI contains safflower equivalent to 500 g and aceglutamide equivalent to 30 g (Ai et al., 2017). According to the corresponding quality control standard, the content of HYSA should not be lower than 0.15 mg/ml and the content of aceglutamide should be in the range of 27–33 mg/ml when detected by HPLC (Ai et al., 2017; Zhou et al., 2021). In ten batches of GHI, our research group found 21 common peaks (Ai et al., 2017). The contents of seven representative ingredients (aceglutamide, uridine, adenosine, guanosine, syringing, hydroxysafflower yellow A, and anhydrosafflor yellow B) were determined (He et al., 2015). The above studies proved that the preparation process of GHI was stable.