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The production of malondialdehyde (MDA) is used as a biomarker to measure the level of lipid peroxidation. Briefly, to evaluate the lipid peroxidation products (MDA), 1.0 mL of 20% trichloroacetic acid and 1.0 mL of 1% thiobarbituric acid reactive substances (TBARS) were added to 100 μL of a supernatant, mixed, then incubated at 100°C for 80 minutes. After cooling on ice, the samples were centrifuged at 3000 rpm for 20 minutes and the absorbance of the supernatant was read at 532 nm (10).
Copyright and License information: ©2016, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences.
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