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2.1. Isolation of the pathogen
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In 2018, a total of seven monoconidial isolates of P. variabilis were collected from Amomum tsao-ko diseased leaves from seven different Amomum tsao-ko orchards in Luchun County (E: 102°17′, N: 22°50′), Honghe Prefecture, Yunnan Province, China. One of seven isolates was tested in this study.
After the isolation of a pure culture, they were stored at 4°C in potato dextrose agar (PDA) media. Before the experiment was conducted, the strains were transferred to PDA media at 27°C for five days until the culture had fully expanded.
Copyright and License information: ©2021 Yiming Zhang ., published by De Gruyter
This work is licensed under the Creative Commons Attribution 4.0 International License.
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