Microorganism and growth condition

Phialophora verrucosa (FMC.2214 strain) isolated from a human patient with CBM was grown on SDA medium. Fungal cells were cultivated for 7 days under constant agitation (130 rpm) at 26°C in 100 ml of Czapek-Dox, a chemically defined medium containing: 3 g sucrose; 0.3 g NaNO₃; 0.05 g MgSO₄.7H₂O; 0.05 g KCl; 0.1 g KH₂PO₄; 0.001 g FeSO₄.7H₂O, pH 5.5. Conidia were obtained after gauze filtration followed by centrifugation (4.000 × g/10 min). Then, conidia were washed three times in 0.85% NaCl and cell density estimated by counting in a Neubauer chamber (Granato et al., 2015). All the experiments with P. verrucosa were conducted under Biosafety Level 2 (BSL-2) conditions.