Pentobarbital sodium (Nembutal, 43 mg/kg, Dainabot Co., Japan) diluted in sterile water for injection was injected into the abdomen to induce general anesthesia, and then the surgical site was sterilized and isolated.
The dorsal portion was incised, and a subcutaneous pouch was formed in both sides. DDM, which was wet with 1.875 w/v% polydeoxyribonucleotide solution, 10 μg/ml, was implanted into the subcutaneous pouch, and the cut was sutured with nylon thread. And antibiotic ointment was applied to prevent postoperative infection.
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