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Cell loading of composite scaffolds
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MSCs and meniscal cells were loaded onto the scaffolds as previously described [13, 25]. Briefly, MSCs and meniscal cells were trypsinized, counted, washed, and resuspended in DMEM-high glucose at a concentration of 1.0 × 106 cells into the composite scaffolds. The cell-scaffold constructs were implanted in the meniscus punch defects without preculture [11].
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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