Published sequences of teleost FXYD proteins (Tipsmark, 2008; Wang et al., 2008; Saito et al., 2010; Tang et al., 2012; Yang et al., 2013; Hu et al., 2014) were obtained from NCBI. All accession numbers are listed in the Table S2. Multiple sequence alignments were performed by Bioedit version 7.2.5 with ClustalW (Hall, 1999). The phylogenetic tree was constructed using MEGA7 with the maximum likelihood method (Kumar et al., 2016), and 1,000 bootstraps were used to test the consistency of the groupings.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.