Alkaline phosphatase (ALP) staining

Rong Hua; Jilong Zou; Ying Ma; Xiaomei Wang; Yao Chen; Yuan Li; Jianyang Du

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Alkaline phosphatase (ALP) staining

RH Rong Hua

JZ Jilong Zou

YM Ying Ma

XW Xiaomei Wang

YC Yao Chen

YL Yuan Li

JD Jianyang Du

This method is extracted from research article: Ann Transl Med, Aug 2021

Psoralidin prevents caffeine-induced damage and abnormal differentiation of bone marrow mesenchymal stem cells via the classical estrogen receptor pathway

DOI: 10.21037/atm-21-3153

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After 21 days of osteogenic differentiation, ALP staining was applied to assess the osteoblastic mineralization of BMSCs. The ALP activity of cell lysates was then detected by spectrophotometric measurement of p-nitrophenol release using an enzymatic colorimetric ALP kit (Roche, Switzerland). The mineralized nodules were quantified by a microplate reader at 420 nm.

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