CML-CP cells from freshly diagnosed patients, B-ALL xenograft cells, Baf3-TEL-ABL1 cell lines, and murine BMCs expressing NUP214-ABL1 oncogene were treated with 5-[3-(4-fluorophenyl)-1-phenylpyrazol-4-yl]imidazolidine-2,4-dione (DPH; Sigma-Aldrich), imatinib and ponatinib (both from Selleck Chemicals) and evaluated as described in supplemental Methods. These studies were approved by the Temple University institutional review board.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.