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Antibodies and protein analysis.

CY Chun-Kit Yuen
CC Ching-Ping Chan
SF Sin-Yee Fung
PW Pei-Hui Wang
WW Wan-Man Wong
HT Hei-Man Vincent Tang
KY Kit-San Yuen
CC Chi-Ping Chan
DJ Dong-Yan Jin
KK Kin-Hang Kok
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Mouse anti-Tax antibody has been described (29, 46). Rabbit anti-TBK1 and anti-phospho-TBK1 (S172) antibodies were purchased from Cell Signaling (Danvers, MA). Mouse anti-V5 antibody was bought from Life Technologies (Grand Island, NY). Mouse anti-HA (Y11), anti-Myc (9E10), and anti-glutathione S-transferase (anti-GST; B14) antibodies were from Santa Cruz Biotechnology (Dallas, TX). Mouse anti-Flag (M2) and rabbit anti-V5 antibodies were from Sigma-Aldrich (St. Louis, MO). Mouse anti-β-tubulin and rabbit anti-GFP antibodies were from Abcam (Cambridge, MA). Western blotting, native gel electrophoresis analysis of IRF3 dimerization, and coimmunoprecipitation were performed as described previously (12, 40, 41).

Copyright and License information:  ©2016, American Society for Microbiology. All Rights Reserved.

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