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Transwell chambers (6.5 mm) with 8-µm pores were used to examine SC migration as described previously [21]. SCs (106 cells/mL) resuspended in 100 µL of DMEM were transferred to the top chamber and allowed to migrate in 5% CO2 into the lower chamber before the addition of 600 µL complete medium. Cells adhering to the bottom surface of each membrane were stained with 0.1% crystal violet, imaged, and counted using a DMR inverted microscope (Leica Microsystems, Bensheim, Germany). The cell migration assays were conducted three times using triplicate wells.
Copyright and License information: The Author(s) ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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