Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Colony-forming Efficiency Assay
Request a Protocol
Ask a
question
Favorite
To compare the clonogenic potential of primary BMSCs derived from BgnFmod KO and WT mice, bone marrow was flushed from femora and tibiae of 9-week old mice, plated in 25 cm2 flasks (106 cell/flask) with growth medium and left to grow untouched for 10d. Cells were then fixed with 100% methanol and following washes with PBS were stained with saturated methyl violet (Gibco) for 30 min. The number of colonies was obtained using light microscopy. Only colonies with more than 30 cells were counted.
Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
