Cell lines and growth

Three human prostate cancer cell lines were used: PC3, DU145, and LNCaP. All three were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). PC3 and LNCaP cells were cultured in RPMI-1640 media containing L-glutamine, 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. DU145 cells were cultured in Minimum Essential Medium Eagle containing Earle’s salts, L-Glutamine, 15% FBS and 1% penicillin/streptomycin. All cell lines were maintained at 37°C and in 95% air and 5% CO₂.