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Total RNA was extracted from the cells, purified and reverse-transcribed using RNeasy (Qiagen) and iScript cDNA synthesis kit (170–8891, Bio-Rad) respectively following manufacturers’ instructions. qRT-PCR was performed using iQ SYBR Green Supermix (170–8886, Bio-Rad). Target genes were normalized to S29 and relative expression data was calculated using the ΔΔCt method. A list of primers used is found in Table S1.
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